3JV2

Crystal Structure of B. subtilis SecA with bound peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97950
Spacegroup name	P 41
Unit cell lengths	106.724, 106.724, 175.605
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
Rwork	0.208
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tf5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	67929
<I/σ(I)>	26.4	2.8
Completeness [%]	98.3	100
Redundancy	5.4	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	0.1M sodium citrate, 8% PEG 4000, 0.1M NaCl, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K