3JV0

Crystal structure of a mutant of RelB dimerization domain(M6)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	105
Detector technology	IMAGE PLATE
Collection date	2006-05-05
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	71.740, 71.740, 61.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.480 - 2.650
Rwork	0.221
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zk9
RMSD bond length	0.007
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.790
High resolution limit [Å]	2.700	2.700
Number of reflections	4224
<I/σ(I)>	12.4	1.5
Completeness [%]	84.0	38
Redundancy	5	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	20 % PEG8000, 0.1 M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K