3JUX
Structure of the translocation ATPase SecA from Thermotoga maritima
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.29 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.533, 119.403, 120.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.100 |
Rwork | 0.227 |
R-free | 0.30300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tf5 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.220 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 17570 | |
<I/σ(I)> | 13.6 | 2.5 |
Completeness [%] | 99.8 | 100 |
Redundancy | 6.8 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M Hepes pH 7.5, 60% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 298K |