3JUK
The Crystal Structure of UDP-glucose pyrophosphorylase complexed with UDP-glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.444, 74.393, 167.123 |
| Unit cell angles | 90.00, 97.91, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| Rwork | 0.230 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3juj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.557 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 54022 | |
| Completeness [%] | 98.8 | 99.8 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M HEPES-Na pH 7.5, 2% PEG 400, 1.5M ammonium sulfate, 10mM UDP-Glucose, 10 mM MgCl2 , VAPOR DIFFUSION, HANGING DROP, temperature 295K |
