3JUB
Human gamma-glutamylamine cyclotransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.956635 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.995, 41.940, 84.012 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.680 - 1.200 |
R-factor | 0.12436 |
Rwork | 0.123 |
R-free | 0.14876 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vkb |
RMSD bond length | 0.023 |
RMSD bond angle | 1.910 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.680 | 1.260 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.058 | 0.323 |
Number of reflections | 40593 | |
<I/σ(I)> | 22.2 | 5.2 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 6.8 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 17.5%(w/v) PEG3350, 0.3M ammonium nitrate, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |