3JU1
Crystal Structure of Enoyl-CoA Hydratase/Isomerase Family Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-28 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 83.982, 83.982, 277.832 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.058 - 2.301 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.23100 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.652 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.4_147)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.060 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 50834 | |
<I/σ(I)> | 13.6 | 5 |
Completeness [%] | 98.3 | 100 |
Redundancy | 11.8 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.1M sodium acetate pH 4.6, 2 M sodium formate, 1/10 V8 protease, VAPOR DIFFUSION, SITTING DROP, temperature 298K |