3JTX

Crystal structure of Aminotransferase (NP_283882.1) from NEISSERIA MENINGITIDIS Z2491 at 1.91 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-14
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97922,0.97936
Spacegroup name	C 2 2 21
Unit cell lengths	112.617, 116.890, 145.326
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.311 - 1.910
R-factor	0.154
Rwork	0.152
R-free	0.18300
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.344
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.311	29.311	1.980
High resolution limit [Å]	1.910	4.100	1.910
Rmerge	0.059	0.024	0.418
Number of reflections	74211	14168	14586
<I/σ(I)>	11.55	32	2.2
Completeness [%]	98.8	97.2	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	20.0000% polyethylene glycol 8000, 0.2000M calcium acetate, 0.1M MES pH 6.0, Additive: 0.001M pyridoxal-5'-phosphate(PLP), NANODROP', VAPOR DIFFUSION, SITTING DROP, temperature 293K