3JT0

Crystal Structure of the C-terminal fragment (426-558) Lamin-B1 from Homo sapiens, Northeast Structural Genomics Consortium Target HR5546A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-06
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97908
Spacegroup name	P 21 21 21
Unit cell lengths	39.437, 72.793, 81.402
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.322 - 2.392
R-factor	0.2383
Rwork	0.238
R-free	0.24500
Structure solution method	Phenix
RMSD bond length	0.007
RMSD bond angle	1.342
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.4_115)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.392	2.400
Rmerge	0.051	0.242
Number of reflections	17107
<I/σ(I)>	5.8	7.7
Completeness [%]	95.8
Redundancy		5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7		Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: 100 mM NH4H2PO4, 100 mM Hepes, 18% PEG3350, VAPOR DIFFUSION, HANGING DROP