3JSY
N-terminal fragment of ribosomal protein L10 from Methanococcus jannaschii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-5 |
Synchrotron site | MAX II |
Beamline | I911-5 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.90778 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.000, 70.080, 97.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.600 |
R-factor | 0.18412 |
Rwork | 0.182 |
R-free | 0.23321 |
Structure solution method | Solved with MAD and refined against native data set |
RMSD bond length | 0.003 |
RMSD bond angle | 0.929 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.056 | 0.380 |
Number of reflections | 50801 | |
<I/σ(I)> | 21.61 | 4.45 |
Completeness [%] | 92.5 | 85 |
Redundancy | 4.7 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | KSCN, PEG4000, NaAcetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |