3JSR
X-Ray structure of All0216 protein from Nostoc sp. PCC 7120 at the resolution 1.8A. Northeast Structural Genomics Consortium target NsR236
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Detector technology | CCD |
| Collection date | 2009-08-05 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.603, 57.603, 97.554 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.800 - 1.800 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.22600 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.280 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.4_115)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.042 | 0.181 |
| Number of reflections | 15602 | |
| <I/σ(I)> | 75.7 | 13.4 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 27.7 | 28.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 293 | 8.64 M potassium acetate, 0.1 M TAPS, pH 9.0, microbatch, temperature 293K |
