3JSO
Classic Protein With a New Twist: crystal structure of a LexA repressor DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-05 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 100.975, 106.935, 65.895 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.530 - 2.290 |
R-factor | 0.268 |
Rwork | 0.266 |
R-free | 0.30600 |
Structure solution method | MAD, MR |
RMSD bond length | 0.005 |
RMSD bond angle | 0.915 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.290 |
Number of reflections | 23570 |
<I/σ(I)> | 21.4 |
Completeness [%] | 72.2 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 6.8 | 292 | 0.1M Bis-tris propane, 0.05M sodium tartrate, 10% glycerol, 15% ethylene glycol, 14% (w/v) PEG3350, pH 6.8, hanging drop, temperature 292K |
1 | hanging drop | 6.8 | 292 | 0.1M Bis-tris propane, 0.05M sodium tartrate, 10% glycerol, 15% ethylene glycol, 14% (w/v) PEG3350, pH 6.8, hanging drop, temperature 292K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Bis-tris propane | ||
10 | 1 | 2 | PEG3350 | ||
2 | 1 | 1 | sodium tartrate | ||
3 | 1 | 1 | glycerol | ||
4 | 1 | 1 | ethylene glycol | ||
5 | 1 | 1 | PEG3350 | ||
6 | 1 | 2 | Bis-tris propane | ||
7 | 1 | 2 | sodium tartrate | ||
8 | 1 | 2 | glycerol | ||
9 | 1 | 2 | ethylene glycol |