3JSE
Crystal structure of archaeal 20S proteasome in complex with mutated P26 activator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-03 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 254.701, 126.099, 180.393 |
Unit cell angles | 90.00, 92.00, 90.00 |
Refinement procedure
Resolution | 19.860 - 2.900 |
R-factor | 0.21271 |
Rwork | 0.212 |
R-free | 0.24126 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yar |
RMSD bond length | 0.017 |
RMSD bond angle | 1.455 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 20.000 | 3.000 |
High resolution limit [Å] | 2.900 | 6.190 | 2.900 |
Rmerge | 0.117 | 0.054 | 0.517 |
<I/σ(I)> | 9.6 | ||
Completeness [%] | 92.9 | 98.7 | 82.2 |
Redundancy | 2.9 | 2.9 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | pH 5.6, VAPOR DIFFUSION, PEG 1000, HANGING DROP, temperature 294K, Sodium Phosphate Citrate, LiSo4, Imidazole, VAPOR DIFFUSION, HANGING DROP |