3JRY
Human Serum albumin with bound Sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Collection date | 2004-01-13 |
| Wavelength(s) | 0.915 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.600, 55.787, 121.344 |
| Unit cell angles | 80.48, 90.75, 63.48 |
Refinement procedure
| Resolution | 27.753 - 2.300 |
| R-factor | 0.2123 |
| Rwork | 0.209 |
| R-free | 0.26810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ao6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.983 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 120.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 51888 | |
| <I/σ(I)> | 13.46 | 3.41 |
| Completeness [%] | 91.1 | 23.8 |
| Redundancy | 1.86 | 1.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 22-25% PEG 3350, 100-200mM ammonium sulphate and 50mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
