3JRV
Structure of poxvirus K7 protein in complex with RNA helicase DDX3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-25 |
Detector | MARRESEARCH |
Wavelength(s) | 0.978 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.889, 69.529, 65.626 |
Unit cell angles | 90.00, 122.17, 90.00 |
Refinement procedure
Resolution | 55.550 - 1.600 |
R-factor | 0.143 |
Rwork | 0.141 |
R-free | 0.19000 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.490 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.600 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.081 | 0.373 |
Number of reflections | 44187 | |
<I/σ(I)> | 35.4 | 3.4 |
Completeness [%] | 97.3 | 84.9 |
Redundancy | 7 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.25 | 291 | 100mM sodium citrate, 15.0% (w/v) PEG 3350, pH 5.25, VAPOR DIFFUSION, HANGING DROP, temperature 291K |