3JRD
Crystal structure of Fis bound to 27 bp DNA F25 containing T2A3 sequence at center
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.296, 93.076, 155.097 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 79.810 - 3.100 |
R-factor | 0.245 |
Rwork | 0.242 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3iv5 |
RMSD bond length | 0.017 |
RMSD bond angle | 2.094 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 90.000 | 90.000 | 3.210 |
High resolution limit [Å] | 3.100 | 6.680 | 3.100 |
Rmerge | 0.186 | 0.152 | 0.666 |
Number of reflections | 10882 | ||
<I/σ(I)> | 7.4 | ||
Completeness [%] | 91.0 | 86.6 | 93.8 |
Redundancy | 3.9 | 3.7 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.2 M Sodium citrate, 0.1 M TRIS-HCl pH 8.5, 38% PEG 400, vapor diffusion, hanging drop, temperature 277K |