3JRC
Crystal structure of Fis bound to 27 bp DNA F29 containing 5 G/Cs at center
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.480, 93.640, 155.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.320 - 3.080 |
R-factor | 0.232 |
Rwork | 0.230 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3iv5 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.215 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 90.000 | 90.000 | 3.210 |
High resolution limit [Å] | 3.100 | 6.680 | 3.100 |
Rmerge | 0.144 | 0.094 | 0.626 |
Number of reflections | 11654 | ||
<I/σ(I)> | 8.4 | ||
Completeness [%] | 93.1 | 99.4 | 61.2 |
Redundancy | 8 | 10.3 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.25 M Sodium citrate, 0.1 M TRIS-HCl pH 8.5, 40% PEG 400, vapor diffusion, hanging drop, temperature 277K |