3JR9
Crystal structure of Fis bound to 27 bp optimal binding sequence F2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.960, 91.690, 154.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.410 - 2.900 |
R-factor | 0.224 |
Rwork | 0.222 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eto |
RMSD bond length | 0.009 |
RMSD bond angle | 1.503 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 90.000 | 90.000 | 3.000 |
High resolution limit [Å] | 2.900 | 6.250 | 2.900 |
Rmerge | 0.165 | 0.133 | 0.668 |
Number of reflections | 14353 | ||
<I/σ(I)> | 6.1 | ||
Completeness [%] | 99.0 | 98.6 | 99.4 |
Redundancy | 3.2 | 3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.2 M Sodium citrate, 0.1 M TRIS-HCl pH 8.5, 36% PEG 400, vapor diffusion, hanging drop, temperature 277K |