3JR4
MutM interrogating an extrahelical G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.343, 95.377, 102.482 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.139 - 2.601 |
R-factor | 0.193 |
Rwork | 0.190 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r2y |
RMSD bond length | 0.005 |
RMSD bond angle | 0.951 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
Rmerge | 0.103 | 0.044 | 0.548 |
Number of reflections | 14207 | ||
<I/σ(I)> | 7.8 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 7.1 | 6.6 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 8K, sodium cacodylate, glycerol, pH 7.0, vapor diffusion, hanging drop, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 8K | ||
2 | 1 | 1 | sodium cacodylate | ||
3 | 1 | 1 | glycerol | ||
4 | 1 | 2 | PEG 8K | ||
5 | 1 | 2 | sodium cacodylate | ||
6 | 1 | 2 | glycerol |