3JQZ
Crystal Structure of Human serum albumin complexed with Lidocaine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.008 |
Spacegroup name | I 41 |
Unit cell lengths | 168.476, 168.476, 97.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.928 - 3.300 |
R-factor | 0.2227 |
Rwork | 0.220 |
R-free | 0.26820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bxe |
RMSD bond length | 0.003 |
RMSD bond angle | 0.715 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 119.523 | 3.500 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.092 | |
Number of reflections | 20507 | |
<I/σ(I)> | 13.04 | 1.16 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.3 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 20-21% polyethylene glycol 6000, 9-10% ethylene glycol, 50/100mM ammonium acetate, 2% 2-methyl-2,4-pentanediol, 50mM Tris-HCl, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |