3JQW
Crystal structure of Clostridium histolyticum colH collagenase collagen-binding domain 3 at 2 Angstrom resolution in presence of calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.001, 64.180, 95.375 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.500 - 2.000 |
| R-factor | 0.17736 |
| Rwork | 0.174 |
| R-free | 0.23323 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o8o |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.567 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.500 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 24837 | |
| <I/σ(I)> | 16.8 | 3.1 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 18.8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG3350, Li2SO4, CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG3350, Li2SO4, CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
