3JQH

Structure of the neck region of the glycan-binding receptor DC-SIGNR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-04
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97945
Spacegroup name	P 4 21 2
Unit cell lengths	34.170, 34.170, 36.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.719 - 2.201
R-factor	0.195
Rwork	0.194
R-free	0.21640
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	0.957
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.719	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.061	0.126
Number of reflections	1283
Completeness [%]	99.9	100
Redundancy	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	Protein solution: 2.8 mg/ml protein, 10 mM Tris-Cl, pH 8.0, and 25 mM NaCl. Reservoir solution: 9% polyethylene glycol 6000, 1.25 M NaCl and 0.1 Bis-Tris, pH 6.5., VAPOR DIFFUSION, HANGING DROP, temperature 291K