3JQ0
Crystal structure of SusD superfamily protein (YP_001299712.1) from Bacteroides vulgatus ATCC 8482 at 1.13 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-08 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 75.804, 75.804, 323.966 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.450 - 1.130 |
R-factor | 0.118 |
Rwork | 0.117 |
R-free | 0.13700 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.673 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.5) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0053) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.450 | 29.450 | 1.160 |
High resolution limit [Å] | 1.130 | 5.050 | 1.130 |
Rmerge | 0.111 | 0.078 | 0.580 |
Total number of observations | 11750 | 30488 | |
Number of reflections | 187091 | ||
<I/σ(I)> | 9.3 | 7.4 | 1.3 |
Completeness [%] | 91.5 | 98.8 | 58.1 |
Redundancy | 5 | 4.4 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 1.60M (NH4)2SO4, 0.1M TRIS pH 8.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |