3JPZ
Crystal Structure of Lombricine Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9764 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.340, 59.550, 85.850 |
| Unit cell angles | 90.00, 105.04, 90.00 |
Refinement procedure
| Resolution | 28.487 - 1.950 |
| R-factor | 0.212 |
| Rwork | 0.208 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Lombricine kinase ADP complex 3jq3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.711 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.790 |
| High resolution limit [Å] | 1.750 | 4.310 | 1.750 |
| Rmerge | 0.131 | 0.092 | 0.378 |
| Number of reflections | 69201 | ||
| <I/σ(I)> | 8.6 | ||
| Completeness [%] | 94.7 | 95.2 | 92.9 |
| Redundancy | 3.4 | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | protein at 30 mg/mL, mixed 1:1 with and equilibrated against 15mM BisTris, 0.2M NaNO3, 1mM DTT, 20% w/v PEG 3350MME, pH 6.8, vapor diffusion, hanging drop, temperature 277K |
