3JD3
Glutamate dehydrogenase in complex with NADH and GTP, open conformation
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
| Method | blot for 3-6 seconds before plunging |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Quantum |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
| Number of Images | 1265 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.6 |
| Software | CTFFIND,Coot,PHENIX,Rosetta,UCSF Chimera,EMAN,RELION |
| Symmetry | D3 |
