3JCN

Structures of ribosome-bound initiation factor 2 reveal the mechanism of subunit association: Initiation Complex I

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK I
Method	Blot for 2-4 seconds before plunging

EM Data Collection

Microscope	FEI POLARA 300
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2
Detector	GATAN K2 (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	4.6
Software	CTFFIND,EMAN,SPARX,SPIDER
Symmetry	C1