3JCI
2.9 Angstrom Resolution Cryo-EM 3-D Reconstruction of Close-packed PCV2 Virus-like Particles
EM specimen
| Vitrification | Yes |
| Instrument | GATAN CRYOPLUNGE 3 |
| Method | Blot for 5 seconds before plunging |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 141 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.9 |
| Software | EMAN,jspr,PHENIX |
| Symmetry | I |
