3JC8
Architectural model of the type IVa pilus machine in a piliated state
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.2 |
| Energy Filter | GIF |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | TOMOGRAPHY |
| Software | IMOD,PEET,TOMO3D |
| Symmetry | C2 |
