3JC1
Electron cryo-microscopy of the IST1-CHMP1B ESCRT-III copolymer
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
| Method | Deposited 3.5 uL sample, blotted 3-6 seconds (0 mm offset) |
EM Data Collection
| Microscope | FEI TITAN KRIOS,FEI TECNAI F20,JEOL 3200FSC |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300,200 |
| Cs (mm) | 2,3.4 |
| Detector | KODAK SO-163 FILM |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 4 |
| Software | CTFFIND,SPIDER,Coot,MDFF,NAMD,Rosetta,UCSF Chimera,EMAN,IHRSR,RELION |
