3JBS
eL6 protein from yeast 60S ribosomal subunit
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
| Method | A 3 uL sample was applied onto a freshly plasma-treated (6 seconds, Gatan Solarus plasma cleaner) holey carbon C-flat grid (Protochips, Inc.), allowing the sample to adsorb for 30 seconds. The sample was then plunge-frozen in liquid ethane using a manual cryo-plunger in ambient atmosphere at 4 degrees C. |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 (4k x 4k) |
| Number of Images | 1833 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.9 |
| Software | Rosetta,UCSF Chimera,FREALIGN |
| Symmetry | C1 |
