3JBO
Cryo-electron microscopy reconstruction of the Plasmodium falciparum 80S ribosome bound to P/E-tRNA
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
| Method | Blot for 4 seconds before plunging. |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.26 |
| Detector | GATAN K2 (4k x 4k) |
| Number of Images | 5734 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.8 |
| Software | CTFFIND,MDFF,Arachnid,RELION |
| Symmetry | C1 |
