3JBO
Cryo-electron microscopy reconstruction of the Plasmodium falciparum 80S ribosome bound to P/E-tRNA
This is a non-PDB format compatible entry.
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
Method | Blot for 4 seconds before plunging. |
EM Data Collection
Microscope | FEI POLARA 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.26 |
Detector | GATAN K2 (4k x 4k) |
Number of Images | 5734 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 5.8 |
Software | CTFFIND,MDFF,Arachnid,RELION |
Symmetry | C1 |