3JBK
Cryo-EM reconstruction of the metavinculin-actin interface
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
| Method | 3 microliters of 0.3 micromolar actin was applied to the grid and incubated for 60 seconds at 25 degrees C. 3 microliters of 10 micromolar MVt was then applied and incubated for 60 seconds. 3 microliters of solution was removed, then an additional 3 microliters of MVt applied. After 60 seconds, 3 microliters of solution was removed, then the grid was blotted for 2 seconds before plunging. |
EM Data Collection
| Microscope | FEI TECNAI 20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 120 |
| Cs (mm) | 2 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
| Number of Images | 671 |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 8.2 |
| Software | CTFFIND,MDFF,UCSF Chimera,EMAN,FREALIGN,SPARX |
