3JBH
TWO HEAVY MEROMYOSIN INTERACTING-HEADS MOTIFS FLEXIBLE DOCKED INTO TARANTULA THICK FILAMENT 3D-MAP ALLOWS IN DEPTH STUDY OF INTRA- AND INTERMOLECULAR INTERACTIONS
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
| Method | Blotting was performed from one side of the grid till a thin sample film on it using Whatman No 42 filter paper, then the grid was immediately plunged under gravity into liquid ethane cooled by liquid nitrogen. Grids were stored under liquid nitrogen. |
EM Data Collection
| Microscope | FEI/PHILIPS CM120T |
| Electron Gun | LAB6 |
| Accelerating Voltage (kV) | 120 |
| Cs (mm) | 2 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 1008 |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 20 |
| Software | Situs,SPIDER |
