3JAW
Atomic model of a microtubule seam based on a cryo-EM reconstruction of the EB3-bound microtubule (merged dataset containing tubulin bound to GTPgammaS, GMPCPP, and GDP)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
| Method | Blot once for 4 seconds before plunging. |
EM Data Collection
| Microscope | FEI TITAN |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 (4k x 4k) |
| Number of Images | 922 |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.9 |
| Software | EMAN,FREALIGN |
