3JAV
Structure of full-length IP3R1 channel in the apo-state determined by single particle cryo-EM
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
| Method | blot once for 3 seconds |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Energy Filter | FEI |
| Detector | GATAN K2 (4k x 4k) |
| Number of Images | 4160 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.7 |
| Software | EMAN,RELION |
| Symmetry | C4 |
