3JAO
Ciliary microtubule doublet
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
| Method | Blot for 3 seconds before plunging |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 1.5 |
| Energy Filter | Gatan GIF Tridem Energy Imaging Filter |
| Detector | GENERIC CCD |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 23 |
| Software | UCSF Chimera,SPIDER |
