3JAO
Ciliary microtubule doublet
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK II |
Method | Blot for 3 seconds before plunging |
EM Data Collection
Microscope | FEI TECNAI F20 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 1.5 |
Energy Filter | Gatan GIF Tridem Energy Imaging Filter |
Detector | GENERIC CCD |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 23 |
Software | UCSF Chimera,SPIDER |