3JAI
Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
| Method | After 30 second wait time, blot for 3 seconds before plunging |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | FEI |
| Detector | FEI FALCON II (4k x 4k) |
| Number of Images | 1472 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.65 |
| Software | CTFFIND,Coot,UCSF Chimera,RELION |
| Symmetry | C1 |
