3JAE
Structure of alpha-1 glycine receptor by single particle electron cryo-microscopy, glycine-bound state
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK III |
Method | Blot for 3.5 seconds before plunging. |
EM Data Collection
Microscope | FEI POLARA 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2 |
Detector | GATAN K2 (4k x 4k) |
Number of Images | 1460 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.9 |
Software | UCSF Chimera,RELION |
Symmetry | C5 |