3JA7
Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution
EM specimen
Vitrification | Yes |
Instrument | GATAN CRYOPLUNGE 3 |
Method | Blot for 7 seconds before plunging |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF |
Detector | GATAN K2 (4k x 4k) |
Number of Images | 839 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.6 |
Software | RELION |
Symmetry | C12 |