3JA7
Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution
EM specimen
| Vitrification | Yes |
| Instrument | GATAN CRYOPLUNGE 3 |
| Method | Blot for 7 seconds before plunging |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF |
| Detector | GATAN K2 (4k x 4k) |
| Number of Images | 839 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.6 |
| Software | RELION |
| Symmetry | C12 |
