3J9Q
Atomic structures of a bactericidal contractile nanotube in its pre- and post-contraction states
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
| Method | Blot time 4 seconds, blot force 1 |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 307 |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.5 |
| Software | IHRSR,RELION |
