3J9B
Electron cryo-microscopy of an RNA polymerase
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
Method | The grids were blotted for 6.5 s with 100% humidity and flash frozen in liquid ethane cooled at liquid nitrogen temperature using an FEI Vitrobot. |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K2 (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4.3 |
Software | UCSF Chimera,EMAN,RELION |
Symmetry | C2 |