3J8V
Cryo-EM reconstruction of quasi-HPV16 complex with H16.14J Fab
EM specimen
| Vitrification | Yes |
| Instrument | GATAN CRYOPLUNGE 3 |
| Method | Blot for 0.7 seconds before plunging. |
EM Data Collection
| Microscope | JEOL 2100 |
| Electron Gun | LAB6 |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
| Number of Images | 385 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 13.9 |
| Software | Situs,UCSF Chimera,Auto3DEM,EMAN |
| Symmetry | I |
