3J8V
Cryo-EM reconstruction of quasi-HPV16 complex with H16.14J Fab
EM specimen
Vitrification | Yes |
Instrument | GATAN CRYOPLUNGE 3 |
Method | Blot for 0.7 seconds before plunging. |
EM Data Collection
Microscope | JEOL 2100 |
Electron Gun | LAB6 |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2 |
Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
Number of Images | 385 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 13.9 |
Software | Situs,UCSF Chimera,Auto3DEM,EMAN |
Symmetry | I |