3J8A
Structure of the F-actin-tropomyosin complex
EM specimen
| Vitrification | Yes |
| Instrument | GATAN CRYOPLUNGE 3 |
| Method | Sample was applied to grid, incubated for 10 seconds, and manually blotted for 3 seconds from the backside with filter paper. |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON II (4k x 4k) |
| Number of Images | 1311 |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.7 |
| Software | Coot,DireX,PHENIX,SPARX |
