3J7H
Structure of beta-galactosidase at 3.2-A resolution obtained by cryo-electron microscopy
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
| Method | Blot for 2 seconds before plunging. |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF |
| Detector | GATAN K2 (4k x 4k) |
| Number of Images | 509 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.2 |
| Software | Coot,UCSF Chimera,EMAN,FREALIGN |
| Symmetry | D2 |
