3J77
Structures of yeast 80S ribosome-tRNA complexes in the rotated and non-rotated conformations (Class II - rotated ribosome with 1 tRNA)
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.01 |
| Detector | FEI FALCON I (4k x 4k) |
| Number of Images | 4754 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 6.2 |
| Software | CTFFIND,CNS,UCSF Chimera,EMAN,FREALIGN,IMAGIC,RSAMPLE |
| Symmetry | C1 |
