3J6S
Cryo-EM structure of Dengue virus serotype 3 at 28 degrees C
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
| Method | Blotted with filter paper for 2 seconds prior to snap freezing |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
| Number of Images | 836 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 6 |
| Software | Coot,MDFF,NAMD,UCSF Chimera,EMAN,MPSA |
| Symmetry | I |
