3J68
Structural mechanism of the dynein powerstroke (pre-powerstroke state)
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
| Method | Blot for 1.5-2.5 seconds before plunging. |
EM Data Collection
| Microscope | FEI TECNAI F30 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF |
| Detector | GENERIC GATAN (2k x 2k) |
3D Reconstruction
| Method | TOMOGRAPHY |
| Resolution (Å) | 30 |
| Software | IMOD,PEET |
| Symmetry | C1 |
