3J5L
Structure of the E. coli 50S subunit with ErmBL nascent chain
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2.7 |
Detector | TVIPS TEMCAM-F416 (4k x 4k) |
Number of Images | 17906 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 6.6 |
Software | UCSF Chimera,SPIDER |
Symmetry | C1 |