3J4S
Helical Model of TubZ-Bt four-stranded filament
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
| Method | Blot 4.5 s before plunging |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.2 |
| Detector | TVIPS TEMCAM-F816 (8k x 8k) |
| Number of Images | 414 |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 6.8 |
| Software | MDFF,SPIDER |
