3J4P
Electron Microscopy Analysis of a Disaccharide Analog complex Reveals Receptor Interactions of Adeno-Associated Virus
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
Method | Both sides of the grid were blotted for 2.5 seconds. |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 120 |
Cs (mm) | 2.7 |
Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
Number of Images | 5207 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4.8 |
Software | UCSF Chimera,Appion,FREALIGN |
Symmetry | I |