3J4K
Cryo-EM structures of the actin:tropomyosin filament reveal the mechanism for the transition from C- to M-state
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
| Method | 3 second blot |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 8 |
| Software | UCSF Chimera,EMAN,MATLAB,SPIDER |
